The Horse in the Floral Print Dress

Buster Keaton's Our Hospitality (1923) includes many great gags, but the following gag has always been one that stood out.

A rowdy slapstick version of the gag, which ends with the horse kicking the villain to the ground, is initiated by dude-turned-cowboy Johnny Arthur in the Tuxedo comedy Honest Injun (1926).  Harold Lloyd effectively reworked the gag for The Kid Brother (1927).

 
While working as a gag man on A Southern Yankee (1948), Keaton handed down the time-tested gag to a next-generation comedian, Red Skelton.